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From inside the next do it analysis, raw research having gas circulate during the throat and gasoline density off acetylene (C 2H2) and helium (He) were acquired at 100 Hz using custom data acquisition software. Data files were saved to disk for later analysis. CardOut data were analyzed using the technique described and validated previously (9). Because calculated CardOut is critically dependent on correct time alignment of gas flow and gas concentration signals, we optimized the time delay by comparing calculated inspired dead space (mass balance of He gas) with known dead space of the breathing valve. This time-delay parameter was relatively constant (±4 ms) among subjects. At each stage of exercise, the arterial to mixed venous O2 content difference, or O2 extraction, was calculated fr...

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